The book explores how surface-functionalized metal nanoparticles can be strategically designed and integrated into chromatographic systems to enhance the separation, identification, and quantitative determination of pharmaceuticals and biologically relevant compounds.

Emphasizing both theoretical foundations and practical applications, the authors examine the physicochemical properties of metal nanoparticles that govern their interactions with analytes, stationary phases, and mobile phases. Attention is given to surface functionalization strategies, including ligand modification, polymer coating, and hybrid nanostructures, and to their influence on selectivity, sensitivity, and chromatographic efficiency. The discussion is grounded in current analytical chemistry principles and supported by representative experimental studies from the literature.

The book systematically addresses the application of functionalized metal nanoparticles across various chromatographic techniques. Case studies illustrate their use in the separation and determination of drugs, metabolites, proteins, and other biological compounds in complex matrices. Method development, optimization, and validation are discussed with a focus on reproducibility and analytical performance. By integrating nanomaterials science with chromatographic analysis, the book highlights new analytical opportunities and outlines future research directions at the intersection of nanotechnology and bioanalytical chemistry. We express our deep gratitude to the authors for the contribution they made to the publication of this manuscript.